BY: THOMAS R COUGHLIN, PHD
Working across 3 universities in a total of 8 different labs, while collaborating with 10 investigators on international and US-based fellowships, knowing where and what to buy for research supplies became second nature.
These ere are the best value and best media supplies for cell culture growth and passaging.
Cell Culture Media
Our Pick – Best Value
Corning’s Dulbecco’s Modified Eagle’s Medium – High glucose (great for musculoskeletal research (mesenchymal lines) and early cell growth. From my understanding these are not different and the Sigma one and this Corning brand are interchangeable for results. It’s not like choosing FBS. In regards to a preference, it’s really easier for you to use the same supplier for easier ordering. VWR is cheaper than Sigma so we recommend that.
Alternative from Sigma
More about this DMEM from Sigma – Sigma Aldrich products are reliable and this line of Dulbecco’s Modified Media was robust and all medias have a color changing dye to indicate when the media is starting to oxidize. They also cell alphaMEM and low glucose MEMs for different cell types.
Dulbecco’s Modified Eagle’s Medium – High glucose (great for musculoskeletal research (mesenchymal lines) and early cell growth.
Trypsin
Our Pick
Sigma Aldrich Trypsin-EDTA solution was always reliable and potent. Only 2.5 mL in a 75 mL flask, and 4 mL in a 175 mL flask was more than enough to trypsinize, while waiting for 5 mins at 37C.
Trypsin-EDTA solution – works great and lasts a long time. Never had to worry about potency.
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