Best Value Media Supplies to Use for Academic and Industry Cell Culture and Passaging Research
BY: THOMAS R COUGHLIN, PHD
Working across 3 universities in a total of 8 different labs, while collaborating with 10 investigators on international and US-based fellowships, knowing where and what to buy for research supplies became second nature.
Without further ado, here are the best value and best media supplies for cell culture growth and passaging.
Cell Culture Media
More about this DMEM from Sigma – Sigma Aldrich products are reliable and this line of Dulbecco’s Modified Media was robust and all medias have a color changing dye to indicate when the media is starting to oxidize. They also cell alphaMEM and low glucose MEMs for different cell types.
Dulbecco’s Modified Eagle’s Medium – High glucose (great for musculoskeletal research (mesenchymal lines) and early cell growth.
Corning’s Dulbecco’s Modified Eagle’s Medium – High glucose (great for musculoskeletal research (mesenchymal lines) and early cell growth.
Sigma Aldrich Trypsin-EDTA solution was always reliable and potent. Only 2.5 mL in a 75 mL flask, and 4 mL in a 175 mL flask was more than enough to trypsinize, while waiting for 5 mins at 37C.
Trypsin-EDTA solution – works great and lasts a long time. Never had to worry about potency.
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